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High-throughput screening of cellulase F mutants from multiplexed plasmid sets using an automated plate assay on a functional proteomic robotic workcell |
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| Authors: | Stephen R Hughes Steven B Riedmuller Jeffrey A Mertens Xin-Liang Li Kenneth M Bischoff Nasib Qureshi Michael A Cotta Philip J Farrelly |
| Affiliation: | (1) United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Bioproducts and Biocatalysis (BBC) Research Unit, 1815 North University Street, Peoria, IL 61604, USA;(2) USDA, ARS, NCAUR, Fermentation Biotechnology (FBT) Research Unit, 1815 North University Street, Peoria, IL 61604, USA;(3) Hudson Control Group, Inc., 10 Stern Avenue, Springfield, NJ 07081, USA |
| Abstract: | BackgroundThe field of plasmid-based functional proteomics requires the rapid assay of proteins expressed from plasmid libraries. Automation is essential since large sets of mutant open reading frames are being cloned for evaluation. To date no integrated automated platform is available to carry out the entire process including production of plasmid libraries, expression of cloned genes, and functional testing of expressed proteins. |
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