Recovery and evolutionary analysis of complete integron gene cassette arrays from Vibrio |
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| Authors: | Yan Boucher Camilla L Nesbø Michael J Joss Andrew Robinson Bridget C Mabbutt Michael R Gillings WFord Doolittle HW Stokes |
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| Institution: | (1) Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, 2109, Australia;(2) Genome Atlantic, Dalhousie University, 5859 University Avenue, Halifax, Nova Scotia, B3H 4H7, Canada;(3) Department of Biological Sciences, Macquarie University, Sydney, NSW, 2109, Australia |
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| Abstract: | Background Integrons are genetic elements capable of the acquisition, rearrangement and expression of genes contained in gene cassettes.
Gene cassettes generally consist of a promoterless gene associated with a recombination site known as a 59-base element (59-be).
Multiple insertion events can lead to the assembly of large integron-associated cassette arrays. The most striking examples
are found in Vibrio, where such cassette arrays are widespread and can range from 30 kb to 150 kb. Besides those found in completely sequenced
genomes, no such array has yet been recovered in its entirety. We describe an approach to systematically isolate, sequence
and annotate large integron gene cassette arrays from bacterial strains. |
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