光动力疗法防治损伤性血管内膜增生的实验研究

目的 :观察光动力疗法 (PDT)防治损伤性血管内膜增生的近期疗效 ,并筛选PDT参数。方法 :首先筛选PDT参数。兔 2 4只 ,按L4 ( 2 3)正交实验方案分为四组 ,处理因素为血啉甲醚 5或 10mg/kg、功率密度 30或 90mw/cm2 、照射时间 15或 30min。球囊法损伤髂总动脉内膜 ,随后进行PDT处理 ,2 1天后取材 ,以中膜与内膜面积的比值为指标分析各处理因素的作用。其次观察PDT疗效。兔 2 4只 ,随机分成 4组 :( 1)单纯损伤组 ;( 2 )PDT对照组 ,内膜损伤后先激光照射 ,后注射血啉甲醚 ;( 3)低PDT剂量组 ,内膜损伤后先注射血啉甲醚 5.0mg/kg ,然后以 30mw/cm2 照射 15min ;( 4 )高PDT剂量组 ,以 90mw/cm2 照射 ,其余同低剂量组。以内膜与中膜面积的比值为指标分析PDT疗效。结果 :预选参数的最优搭配为 :功率密度为 30mw/cm2 ,照射时间为 15min ,血啉甲醚剂量为 5mg/kg。低剂量组与高剂量组的S1/S2 比值分别比单纯损伤组低 71.9% (P <0 .0 1)和 59.4 % (P <0 .0 5) ,比PDT对照组低 63.1% (P <0 .0 1)和 4 6.5% (P <0 .0 5)。结论 :经皮血管内光动力疗法可能成为防治损伤性血管内膜增生的有效方法。

Prevention of Arterial Intimal Hyperplasia after Injury by Photodynamic Therapy

Objective:To investigate the rationality a nd validity of photodynamic therapy(PDT) in the prevention of arterial intimal h yperplasia after acute injury.Results:Ratios of the media/in tima for groups 1,2,3,and 4 were 4.6±1.2,3.3±0.8,2.1±0.3,and 2.4±0.5,resp ectively. The ratio of groups 1 was significantly increased when compared with group 2,3 and group 4(P＜0.01).Ratio s of the intima/media were significantly reduced in group 3 and 4 when compared with group 1 and group 2(P＜0.01).Conclusion:The best parame ters of PDT with HMME as the photosensitizer is :irradiated with 30?mW/cm2 fo r 15?min,and HMME of 5?mg/kg,and PDT was effective in reducing intimal hyperpl asia following arterial injury with the selected parameters.