Analysis of interactions between carbohydrates and proteins using fluorescent labeling and SDS-PAGE |
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| Authors: | Huang Gang L Wong Kongliang |
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| Affiliation: | College of Chemistry, Chongqing Normal University, Chongqing 400047, China. huangdoctor226@163.com |
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| Abstract: | A rapid method for the analysis of carbohydrate-protein interactions by using fluorescent labeling and SDS-PAGE was developed. The N-acetyl-beta-D-glucosamine-WGA complex and alpha-D-mannose-Con A complex were labeled with 8-aminonaphthalene-1,3,6-trisulfonate (ANTS). The protein band displaying fluorescence with ultraviolet illumination was seen after SDS-PAGE. |
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