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Partial immunochemical identity between (Na+ + K+)-ATPase and a membrane component extractable with chloroform: methanol
Authors:J S Addis  K M Tenbarge  R J Barrnett
Abstract:An IgG fraction prepared from an antiserum against a holoenzyme preparation of (Na+ + K+)-ATPase precipitated a single antigen when samples of holoenzyme were subjected to crossed immunoelectrophoresis but precipitated an additional, immunochemically-related antigen when a plasma membrane-enriched fraction was subjected to crossed immunoelectrophoresis under the same conditions. The immunochemically-related antigen could be extracted from the plasma membrane fraction with CHCl3:CH3OH.
Keywords:(Na+ + K+)-ATPase  plasma membrane  avian salt gland  immunochemistry
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