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Immobilization of fructosyltransferase by chitosan and alginate for efficient production of fructooligosaccharides
Institution:1. Microbial Technology Laboratory, Department of Zoology, Dr. Harisingh Gour University, Sagar, MP, India;2. Department of Applied Microbiology and Biotechnology, Dr. Harisingh Gour University, Sagar, MP, India;1. Department of Exact and Land Sciences, Integrated Regional University of the Alto Uruguai and Missões (URI), Av Sete de Setembro 1621, Postal Code: 99700-000, Erechim, Rio Grande do Sul, Brazil;2. Department of Bioscience, Multidisciplinary Institute on Health (IMS), Federal University of Bahia campus Anísio Teixeira (CAT/UFBA), R. Rio de Contas, 80, Candeias, Postal Code: 45.029-094, Vitória da Conquista, Bahia, Brazil;3. Department of Engineering and Computer Science, Integrated Regional University of the Alto Uruguai and Missões (URI), Av Sete de Setembro 1621, Postal Code: 99700-000, Erechim, Rio Grande do Sul, Brazil;4. Department of Agricultural Science, Integrated Regional University of the Alto Uruguai and Missões (URI), Av Sete de Setembro 1621, Postal Code: 99.700-000, Erechim, Rio Grande do Sul, Brazil;5. Department of Food Engineer, Faculty of Food Engineer (FEA), University of Campinas (UNICAMP), R. Monteiro Lobato, 80, Cidade Universitária, Postal Code: 13083-862. Campinas, São Paulo, Brazil;1. Faculté Polytechnique de Mons, Université de Mons, 20 Place du Parc, 7000, Mons, Belgium;2. Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710–057, Braga, Portugal;1. Department of Food Environmental and Nutritional Science (DeFENS), University of Milan, Via Mangiagalli, 20133 Milan, Italy;2. Department of Pharmaceutical Sciences (DISFARM), University of Milan, Via Mangiagalli 25, 20133 Milan, Italy;3. Instituto de Catálisis y Petroleoquímica, CSIC, 28049 Madrid, Spain;1. Group of Bioprocesses, Food Research Department, School of Chemistry, University Autonomous of Coahuila, 25280 Saltillo, Coahuila, Mexico;2. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, Netherlands;3. IBB – Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal;1. College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, Guangdong, China;2. Centre for Food Research and Innovation, Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New Zealand
Abstract:The effective system of reusing mycelial fructosyltransferase (FTase) immobilized with two polymers, chitosan and alginate were evaluated for continuous production of fructooligosaccharides (FOS). The alginate beads were successfully developed by maintaining spherical conformation of using 0.3% (w/v) sodium alginate with 0.1% (w/v) of CaCl2 solution for highest transfructosylating activity. The characteristics of free and immobilized FTase were investigated and results showed that optimum pH and temperature of FTase activity were altered by immobilized materials. A successive production of FOS by FTase entrapped alginate beads was observed at an average of 62.96% (w/w) up to 7 days without much losing its activity. The data revealed by HPLC analysis culminate 67.75% (w/w) of FOS formation by FTase entrapped alginate beads and 42.79% (w/w) by chitosan beads in 36 h of enzyme substrate reaction.
Keywords:Immobilization  Fructosyltransferase  Fructooligosaccharides  Alginate  Chitosan
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