Microalga Ochromonas danica fermentation and lipid production from waste organics such as ketchup |
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| Affiliation: | 1. Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325-3906, United States;2. Creative Fuels, LLC, 1093 Foxglove Circle, Cuyahoga Falls, OH 44223, United States;1. The Joint Graduate School of Energy and Environment (JGSEE), King Mongkut''s University of Technology Thonburi, Thungkru, Bangkok 10140, Thailand;2. Biochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetic Engineering and Biotechnology at King Mongkut''s University of Technology Thonburi, Bangkhuntien, Bangkok 10150, Thailand;3. School of Bioresources and Technology, King Mongkut''s University of Technology Thonburi, Bangkhuntein, Bangkok 10150, Thailand;4. Department of Agro-Industrial, Food, and Environment Technology, Faculty of Applied Science, King Mongkut''s University of Technology North Bangkok, Bangkok 10800, Thailand;5. The Biosensor and Bioelectronics Technology Centre, King Mongkut''s University of Technology North Bangkok, Bangkok 10800, Thailand;1. School of Earth and Environmental Sciences, College of Natural Sciences, Seoul National University, Seoul 151-747, Republic of Korea;2. Advanced Institutes of Convergence Technology, Suwon, Gyeonggi-do 443-270, Republic of Korea;3. Biological Institute, Section of Marine Biology, University of Copenhagen, Universitetsparken 4, DK-2100 Copenhagen K, Denmark;1. Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan;2. Advanced Coating Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan;3. Department of Marine Biology and Environmental Science, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki, Miyazaki 889-2192, Japan;1. Faculty of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran;2. Research Center for New Technologies in Life Science Engineering, University of Tehran, Tehran, Iran;3. Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran, Iran;4. Department of Mechanical Engineering, University of Kashan, Kashan, Iran;1. Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran;2. Department of Plant Ecophysiology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran;3. Iranian National Institute for Oceanography and Atmospheric Science, Persian Gulf Center, Boushehr, Iran;4. Sepanir Oil and Gas Energy Eng. Co., Tesko Phase CO., Asaluyeh, Iran |
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| Abstract: | Ketchup is highly viscous and contains high concentrations of carbohydrates. These properties make waste ketchup more costly to treat prior to disposal. In this study ketchup was used to grow Ochromonas danica, a phagotrophic microalga that can produce and accumulate intracellular lipids using soluble or particulate organic substrates. Effects of the following factors on high-density O. danica fermentation were evaluated: nitrogen source, pH, temperature, agitation speed, aeration method, dissolved oxygen concentration, and nutrient feeding strategy. Under the optimized conditions the alga grew with a doubling time of about 10 h. Cell yield and intracellular lipid yield from the consumed ketchup carbohydrate were 40% and 18%, respectively. The intracellular lipid content could be raised to 40%. The developed process may also be applied to effectively convert other organic wastes to algal lipids, for use as, e.g., biofuel feedstock. |
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| Keywords: | Lipids Algae Biofuel Ketchup |
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