A transcriptomic analysis on gene expressions in the infective third and pathogenic fifth larval stages of Angiostrongylus cantonensis |
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Affiliation: | 1. Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Kwei-Shan, Tao-Yuan 333, Taiwan;2. Department of Parasitology, College of Medicine, Chang Gung University, Kwei-Shan, Tao-Yuan 333, Taiwan;3. Bioinformatics Center, Chang Gung University, Kwei-Shan, Tao-Yuan 333, Taiwan;4. Department of Parasitology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan;5. Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Kwei-Shan, Tao-Yuan 333, Taiwan;1. Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Biopolis, 136648 Singapore;2. Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore;1. Laboratorio de Enzimología de Parásitos, Facultad de Ciencias, Universidad de Los Andes, La Hechicera, Mérida 5101, Venezuela;2. Laboratorio de Fisiología de Parásitos, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas, Caracas 1020-A, Venezuela;1. Department of Clinical and Experimental Medicine, University of Sassari, Italy;2. Department of Surgical, Microsurgical and Medical Sciences, University of Sassari, Italy;3. Department of Clinical and Molecular Biomedicine, University of Catania, Italy;1. Laboratory of Diagnostics Development, China Animal Health and Epidemiology Center, 369 Nanjing Road, Qingdao, Shandong 266032, China;2. College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, Jiangsu 225009, China;3. China Institute of Veterinary Drug Control, 8 Zhongguanchun South Street, Beijing 100081, China;1. School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru;2. Facultad de Agronomía y Zootecnia, Universidad Nacional San Antonio Abad del Cusco, Cusco, Peru;3. Parasitic Diseases Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, GA, United States;1. Department of Biology, Zaporizhzhia National University, Zhukovskogo 66, 69063 Zhaporizhzhia, Ukraine;2. UPMC Univ Paris 06, UMR 7232, BIOM, Observatoire Océanologique, Banyuls/Mer, France;3. CNRS, UMR 7232, BIOM, Observatoire Océanologique, Banyuls/Mer, France |
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Abstract: | Although Angiostrongylus cantonensis is a parasite of rats, it is an important etiologic agent of eosinophilic meningitis and eosinophilic meningoencephalitis in humans. This study was designed to compare the gene expression in the third- and fifth-stage (L3 and L5) by analysis of expressed sequence tags (ESTs). After removing low quality sequences, vector trimming, clustering and contig assembly, there remained 1437 clusters (285 contigs and 1152 singletons). Among these clusters, 779 (54.2%) showed significant similarity to the known proteins in the non-redundant protein database of GenBank (E-value < 1 × 10− 10) and species of the best hit sequences mainly belonged to nematodes. These clusters included 869 (60.5%) that were entirely comprised of ESTs from L3 (L3-biased clusters), 518 (36.0%) entirely from L5 (L5-biased clusters) and 50 (3.5%) from both stages (stage-shared clusters). Functional annotations by the Gene Ontology (GO) comparing with the eukaryotic clusters of orthologous groups of proteins (KOG) indicate that the L3-biased clusters significantly related to metabolism and the L5-biased clusters to growth, development, sexual differentiation and reproduction. Moreover, L3 were found to have expressions of metalloproteases, aspartic proteases, and cysteine proteases whereas expressions of cysteine, aspartic and serine proteases were revealed in L5. The results indicate that earlier developmental stages of nematodes may have a gene expression profile towards metabolism and later stages towards growth and development. |
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