Effects of redox potential control on succinic acid production by engineered Escherichia coli under anaerobic conditions |
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| Institution: | 1. Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, People’s Republic of China;2. Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People’s Republic of China;3. Gaogang Science and Technology Bureau, Taizhou, People’s Republic of China;1. Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, China;2. University of Chinese Academy of Sciences, China;3. College of Biotechnology, Tianjin University of Science & Technology, China;1. Department of Bioengineering, Rice University, Houston, TX 77005, USA;2. Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, USA;3. Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005, USA;1. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea;2. BioInformatics Research Center, KAIST, Daejeon 34141, Republic of Korea;3. BioProcess Engineering Research Center, KAIST, Daejeon 34141, Republic of Korea;1. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus program), BioProcess Engineering Research Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea;2. Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea |
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| Abstract: | The effects of oxido-reduction potential (ORP) control on succinic acid production have been investigated in Escherichia coli LL016. In LL016, two CO2 fixation pathways were achieved and NAD+ supply was enhanced by co-expression of heterologous pyruvate carboxylase (PYC) and nicotinic acid phosphoribosyltransferase (NAPRTase). During anaerobic fermentation, cell growth and metabolite distribution were changed with redox potential levels in the range of ?200 to ?400 mV. From the results, the ORP level of ?400 mV was preferable, which resulted in the high succinic acid concentration (28.6 g/L) and high succinic acid productivity (0.33 g/L/h). Meanwhile, the yield of succinic acid at the ORP level of ?400 mV was 39% higher than that at the ORP level of ?200 mV. In addition, a higher NADH/NAD+ ratio and increased enzyme activities were also achieved by regulating the culture to a more reductive environment, which further enhanced the succinic acid production. |
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| Keywords: | Succinic acid Anaerobic fermentation ORP |
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