Direct bioconversion of d-xylose to 1,2,4-butanetriol in an engineered Escherichia coli |
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| Institution: | 2. Division of Bioscience and Bioinformatics, Myongji University, Myongji-ro 116, Nam-dong, Cheoin-gu, Yongin-si, Gyeonggi-do 449-728, Republic of Korea;1. Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;2. Tsinghua Innovation Center in Dongguan, Dongguan 523808, China;1. Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455, USA;2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;3. College of Life Science, Shanxi Normal University, Linfen 041004, China;1. Department of Bioengineering, University of Illinois at Urbana-Champaign, 1304 W. Springfield Avenue, Urbana, IL 61801, USA;2. Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;3. Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;1. Department of Biology and Program in Molecular Plant Biology, Colorado State University, Fort Collins, CO 80523-1878, USA;2. Proteomics and Metabolomics Facility, Colorado State University, Fort Collins, CO 80523, USA |
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| Abstract: | The compound 1,2,4-butanetriol (BT) is a valuable chemical used in the production of plasticizers, polymers, cationic lipids and other medical applications, and is conventionally produced via hydrogenation of malate. In this report, BT is biosynthesized by an engineered Escherichia coli from d-xylose. The pathway: d-xylose → d-xylonate → 2-keto-3-deoxy-d-xylonate → 3,4-dihydroxybutanal → BT, was constructed in E. coli by recruiting a xylose dehydrogenase and a keto acid decarboxylase from Caulobacter crescentus and Pseudomonas putida, respectively. Authentic BT was detected from cultures of the engineered strain. Further improvement on the strain was performed by blocking the native d-xylose and d-xylonate metabolic pathways which involves disruption of xylAB, yjhH and yagE genes in the host chromosome. The final construct produced 0.88 g L?1 BT from 10 g L?1 d-xylose with a molar yield of 12.82%. By far, this is the first report on the direct production of BT from d-xylose by a single microbial host. This may serve as a starting point for further metabolic engineering works to increase the titer of BT toward industrial scale viability. |
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| Keywords: | 1 2 4-Butanetriol Metabolic engineering |
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