Using low temperature to balance enzymatic saccharification and furan formation during SPORL pretreatment of Douglas-fir |
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Institution: | 1. Department of Pharmaceutical Technology, University of Szeged, Eötvös utca 6., H-6720 Szeged, Hungary;2. Richter Gedeon Ltd., Gyömr?i út 17-19., H-1103 Budapest, Hungary;3. Laboratory of Nuclear Chemistry, Eötvös Loránd University, P.O. Box 32, H-1518 Budapest 112, Hungary;4. Department of Applied Informatics, University of Szeged, Boldogasszony sgt. 6., H-6720 Szeged, Hungary |
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Abstract: | Comparing analytical results for Sulfite Pretreatment to Overcome the Recalcitrance of Lignocelluloses (SPORL) of Douglas-fir (Pseudotsuga menziesii) at two different temperatures shows that the apparent activation energy of sugar degradation is higher than that of hemicellulose hydrolysis, approximately 161 kJ/mole versus 100 kJ/mole. Thus, one can balance the production of degradation products against hemicellulose hydrolysis and therefore the enzymatic saccharification efficiency of the resultant substrate by changing pretreatment temperature and duration. Specifically, pretreatment at 165 °C for 75 min significantly reduced furan formation compared with the pretreatment at 180 °C for 30 min while maintaining the same pretreatment severity and therefore the same substrate enzymatic digestibility (SED). Obtaining high SED with Douglas-fir is also limited by lignin content. Fortunately, the bisulfite in SPORL provides delignification activity. By combining kinetic models for hemicelluloses hydrolysis, sugar degradation, and delignification, the performance of pretreatment can be optimized with respect to temperature, duration, acid, and bisulfite loading. The kinetic approach taken in this study is effective to design viable low temperature pretreatment processes for effective bioconversion of lignocelluloses. |
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Keywords: | Low temperature pretreatment Enzymatic hydrolysis/saccharification Sugar degradation/inhibitor formation Kinetics Combined severity/hydrolysis factor Woody biomass |
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