濒危药用植物掌裂兰根部内生真菌和根际土真菌多样性分析

【背景】植物内生真菌对宿主植物促生长、抗旱和增强抗病能力等方面有着重大的研究和利用价值，尤其对兰科植物的生长起到重要的作用。【目的】通过对掌裂兰根部内生真菌和根际土真菌多样性进行系统分析，掌握掌裂兰根部内生真菌与根际土真菌群落结构，为进一步探究掌裂兰植物与真菌共生规律提供参考。【方法】采用Illumina MiSeq高通量测序技术分析掌裂兰根部内生真菌和根际土真菌多样性。【结果】掌裂兰根部内生真菌隶属于7门89属，优势菌属为瘤菌根菌属(Epulorhiza)(16.93%)、头梗霉属(Cephaliophora)(10.41%)、酵母属(Saccharomyces)(5.73%)、角担菌属(Ceratobasidium)(5.32%)和镰刀菌属(Fusarium)(5.12%)，其中Epulorhiza和Ceratobasidium为兰科植物菌根真菌；根际土真菌隶属于11门269属，优势菌属为镰刀菌属(Fusarium)(8.09%)、丛赤壳属(Neonectria)(6.79%)、Plectosphaerella (3.39%)和被孢霉属(Mortierella)(3.01%)。通过...

Diversity of fungi in the roots and rhizosphere soil of the endangered medicinal plant Dactylorhiza hatagirea

Background] Endophytic fungi capable of promoting plant growth, enhancing plant tolerance to drought, and improving plant resistance to diseases have significant research and utilization values, especially for the growth of orchid plants. Objective] To systematically analyze the diversity and reveal the community structure of fungi in the roots and rhizosphere soil of Dactylorhiza hatagirea, so as to provide a reference for further exploring the associations between D. hatagirea and fungi. Methods] The Illumina Miseq high-throughput sequencing was employed to analyze the diversity of fungi in the roots and rhizosphere soil of D. hatagirea. Results] The endophytic fungi in the roots of D. hatagirea belonged to 89 genera of 7 phyla, with the dominant genera being Epulorhiza (16.93%), Cephaliophora (10.41%), Saccharomyces (5.73%), Ceratobasidium (5.32%), and Fusarium (5.12%), among which Epulorhiza and Ceratobasidium were mycorrhizal fungi in orchid plants. The fungi in the rhizosphere soil belonged to 269 genera of 11 phyla, with the dominant genera being Fusarium (8.09%), Neonectria (6.79%), Plectosphaerella (3.39%), and Mortierella (3.01%). The diversity of fungi in the rhizosphere soil of D. hatagirea was significantly higher than that in the roots. Conclusion] This study revealed the fungal composition in the roots and rhizosphere soil of D. hatagirea in Inner Mongolia, enriching the endophytic fungi resources of this medicinal plant. The findings help to promote the research on endophytic microorganisms in the medicinal plants with limited resources, alleviate the shortage of Chinese medicinal herb resources, and protect and reasonably develop wild medicinal plant resources.