Virus-induced gene silencing: A versatile tool for discovery of gene functions in plants |
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| Authors: | Arunima Purkayastha Indranil Dasgupta |
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| Institution: | 1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei, China;2. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, Guangdong, China;3. Graduate University of the Chinese Academy of Sciences, Beijing 100039, China;4. Institute of Chinese Materia Medica, Chinese Academy of Chinese Medical Science, Beijing 100700, China;1. Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan;2. Frontier Research Laboratories, Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Kanagawa 210-8681, Japan;3. Agri-Innovation Center, Iwate University, Morioka, Iwate 020-8550, Japan;1. Experimental Farm, Graduate School of Agriculture, Kyoto University, Takatsuki 569-0096, Japan;2. Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan;3. Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan |
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| Abstract: | Virus-induced gene silencing (VIGS) is a technology that exploits an antiviral defense mechanism in plants as a tool for plant reverse genetics. VIGS circumvents the need for plant transformation, is methodologically simple and yields rapid results. Various VIGS vectors have been developed and have helped to unravel the functions of genes involved in processes such as disease resistance, abiotic stress, cellular signaling and secondary metabolite biosynthesis. |
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