The Jasmonic Acid Signalling Pathway Restricts the Development of the Arbuscular Mycorrhizal Association in Tomato |
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Authors: | María José Herrera-Medina María Isabel Tamayo Horst Vierheilig Juan Antonio Ocampo José Manuel García-Garrido |
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Institution: | 1.Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC,Granada,Spain;2.Institut für Pflanzenschutz, Department für Angewandte Pflanzenwissenschaften und Pflanzenbiotechnologie,Universit?t für Bodenkultur Wien,Wien,Austria |
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Abstract: | The role of the jasmonate signalling pathway in modulating the establishment of the arbuscular mycorrhiza (AM) symbiosis between
tomato plants and Glomus intraradices fungus was studied. The consequences of AM formation due to the blockage of the jasmonate signalling pathway were studied
in experiments with plant mutants impaired in JA perception. The tomato jai-1 mutant (jasmonic acid insensitive 1) failed to regulate colonization and was more susceptible to fungal infection, showing
accelerated colonization. The frequency and the intensity of fungal colonization were greatly increased in the jai-1 insensitive mutant plants. In parallel, the systemic effects on mycorrhization due to the activation of the jasmonate signalling
pathway by foliar application of MeJA were evaluated and histochemical and molecular parameters of mycorrhizal intensity and
efficiency were measured. Histochemical determination of fungal infectivity and fungal alkaline phosphatase activity reveal
that the systemic application of MeJA was effective in reducing mycorrhization and mainly affected fungal phosphate metabolism
and arbuscule formation, analyzed by the expression of GiALP and the AM-specific gene LePT4, respectively. The results of the present study clearly show that JA participates in the susceptibility of tomato to infection
by arbuscular mycorrhizal fungi, and it seems that arbuscular colonization in tomato is tightly controlled by the jasmonate
signalling pathway. |
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Keywords: | Tomato Arbuscular mycorrhiza Jasmonic acid MeJA-insensitive mutants |
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