Distribution of actin filaments in fertilized egg of the ascidian Ciona intestinalis |
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| Authors: | T Sawada K Osanai |
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| Affiliation: | 1. Unidad de Infectología, Hospital Nacional Guillermo Almenara Irigoyen – EsSalud, Lima, Perú;2. Unidad de Genómica, Laboratorios de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Perú;3. Facultad de Medicina Humana, Universidad Peruana Cayetano Heredia, Lima, Perú;4. Laboratorio de Micología Clínica, Instituto de Medicina Tropical Alexander von Humboldt – Universidad Peruana Cayetano Heredia, Lima, Perú;1. Institute of Zoology, University of Innsbruck, Austria;2. Center for Molecular Biosciences, University of Innsbruck, Austria;3. Department of Zoology, University of British Columbia, Vancouver, Canada;4. Dept. of Biology, McMaster University, Hamilton, Canada;5. Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, USA;1. School of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW 2308, Australia;2. Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region, China;3. New South Wales Department of Primary Industries, Port Stephens Fisheries Institute, Taylors Beach, NSW 2316, Australia |
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| Abstract: | ![]()
Microfilaments in the contracting cortex during the bipolar ooplasmic segregation of Ciona intestinalis eggs were examined by two methods, staining with fluorescent phalloidin and decoration with myosin subfragment 1 (S1). Fluorescent (Fl-)phalloidin revealed prominent fluorescence in the contracting cortex between the surface constriction and the vegetal pole of fertilized eggs. The animal pole did not stain. After extraction in Triton X-100, the cortex appeared as a thin layer that easily separated from cytoplasmic mass, especially at the contracting stage after fertilization. This layer also stained strongly with Fl-phalloidin. S1-decoration confirmed that actin filaments were abundant in the thin layer of Triton-extracted cortex. The actin filaments are considered to compose a contractile network covering the vegetal side of the constriction. |
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