Detailed comparison between the substrate specificities of two angular dioxygenases, dibenzofuran 4,4a-dioxygenase from Terrabacter sp. and carbazole 1,9a-dioxygenase from Pseudomonas resinovorans |
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Authors: | Terufumi Takagi Hideaki Nojiri Takako Yoshida Hiroshi Habe Toshio Omori |
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Institution: | (1) Biotechnology Research Center, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan |
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Abstract: | The preferred substrates in angular dioxygenation, monooxygenation, and lateral dioxygenation by dibenzofuran 4,4a-dioxygenase (DFDO) from Terrabacter sp. strain DBF63 and carbazole 1,9a-dioxygenase (CARDO) from Pseudomonas resinovorans strain CA10 are shown to be distinctly different. The preferred oxygenation reactions suggest that DFDO evolved from a polycyclic aromatic hydrocarbon dioxygenase and that its most preferred substrates were fluorene and 9-fluorenone. The angular dioxygenases involved in the degradation pathway of dibenzofuran (dioxin) and fluorene are closely related in function, while CARDO is a novel enzyme not only phylogenetically but also functionally. |
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Keywords: | angular dioxygenation lateral dioxygenation monooxygenation substrate specificity |
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