Structural and genetic characterization of Escherichia coli O99 antigen |
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Authors: | Andrei V Perepelov Dan Li Bin Liu Sof'ya N Senchenkova Dan Guo Sergei D Shevelev Alexander S Shashkov Xi Guo Lu Feng Yuriy A Knirel & Lei Wang |
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Institution: | Laboratory of Microbial Technology, Department of Bioscience and Biotechnology, Division of Microbial Science and Technology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan;;Department of Health Promotion and Human Behavior, Kyoto University Graduate School of Public Health, Kyoto, Japan;;Department of Preventive Medicine, Division of Social Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan;and;Laboratory of Functional Food Design, Department of Functional Metabolic Design, Bio-Architecture Center, Kyushu University, Fukuoka, Japan |
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Abstract: | The influence of antibiotic exposure in the early postnatal period on the development of intestinal microbiota was monitored in 26 infants including five antibiotic-treated (AT) subjects orally administered a broad-spectrum antibiotic for the first 4 days of life and three caesarean-delivered (CD) subjects whose mothers were intravenously injected by the similar type of antibiotics in the same period. The faecal bacterial composition was analysed daily for the first 5 days and monthly for the first 2 months. Terminal restriction fragment length polymor-phisms in the AT subjects showed less diversity with the attenuation of the colonization of some bacterial groups, especially in Bifidobacterium and unusual colonization of Enterococcus in the first week than the control antibiotic-free infants (AF, n =18). Quantitative real-time PCR showed overgrowth of enterococci (day 3, P =0.01; day 5, P =0.003; month 1, P =0.01) and arrested growth of Bifidobacterium (day 3, P =0.03) in the AT group. Furthermore, after 1 month, the Enterobacteriaceae population was markedly higher in the AT group than in the AF group (month 1, P =0.02; month 2, P =0.02). CD infants sustained similar, although relatively weaker, alteration in the developing microbiota. These results indicate that antibiotic exposure at the beginning of life greatly influences the development of neonatal intestinal microbiota. |
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Keywords: | neonatal intestinal microbiota antibiotics quantitative real-time PCR terminal restriction fragment length polymorphism caesarian delivery |
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