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New miRNA labeling method for bead-based quantification
Authors:Alberto Biscontin  Silvia Casara  Stefano Cagnin  Lucia Tombolan  Angelo Rosolen  Gerolamo Lanfranchi  Cristiano De Pittà
Affiliation:1.Department of Biology and CRIBI Biotechnology Centre,Università degli Studi di Padova,Padova,Italy;2.Clinica di Oncoematologia Pediatrica, Azienda Ospedaliera-Università degli Studi di Padova,Padova,Italy
Abstract:

Background  

microRNAs (miRNAs) are small single-stranded non-coding RNAs that act as crucial regulators of gene expression. Different methods have been developed for miRNA expression profiling in order to better understand gene regulation in normal and pathological conditions. miRNAs expression values obtained from large scale methodologies such as microarrays still need a validation step with alternative technologies.
Keywords:
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