Effects of Bothrops asper snake venom on the expression of cyclooxygenases and production of prostaglandins by peritoneal leukocytes in vivo,and by isolated neutrophils and macrophages in vitro |
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| Authors: | Vanessa Moreira José María Gutiérrez Rafaela Bacci Amaral Stella Regina Zamunér Catarina de Fátima Pereira Teixeira |
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| Affiliation: | 1. Laboratorio de Farmacologia, Instituto Butantan, Av. Vital Brasil, 1500, CEP 05503-900, Sao Paulo, SP, Brazil;2. Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica;1. Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José 11501, Costa Rica;2. Instituto de Biomedicina de Valencia, CSIC, Jaume Roig 11, 46010 Valencia, Spain;1. Department of Environmental Systems Science, ETH Zurich, 8092 Zurich, Switzerland;2. Research School of Earth Sciences, Australian National University, Canberra, Australia;3. Australian Research Council Centre of Excellence for Climate System Science, Canberra, Australia;1. Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica (CeTroGen), Núcleo de Hospital Universitário (NHU), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil;2. Programa de Mestrado em Farmácia, Centro de Ciências Biológicas e da Saúde (CCBS), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil;3. Programa de Pós-graduação em Biotecnologia e Biodiversidade – Rede Pró Centro-Oeste, Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil;4. Programa de Pós-graduação em Saúde e Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina “Dr. Hélio Mandetta” (FAMED), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil;5. Departamento de Química, Universidade Federal do Paraná (UFPR), Curitiba, Paraná, Brazil;6. Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados (UFGD), Dourados, Mato Grosso do Sul, Brazil;7. Centro de Ciências Biológicas e da Saúde (CCBS), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil;1. School of Medicine and Public Health, University of Newcastle, NSW, Australia;2. Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka;1. Department of Otolaryngology, International University of Health and Welfare, Mita Hospital, Shinjuku-ku, Tokyo, Japan;2. Department of Otolaryngology, Tokyo Medical University, Shinjuku-ku, Tokyo, Japan;3. Tokyo Voice Center, International University of Health and Welfare, Sanno Hospital, Minato-Ku, Tokyo, Japan |
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| Abstract: | In this study, the ability of Bothrops asper snake venom (BaV) to increase the production of prostaglandins PGE2 and PGD2 was assessed in a mouse model in vivo and in inflammatory cells in vitro. In addition, the expressions of COX-1 and COX-2 were assessed. BaV induced an increment in the in vivo synthesis of PGE2 and PGD2, together with an enhanced expression of COX-2, but not of COX-1. However, enzymatic activities of COX-1 and COX-2 were increased. Incubation of isolated macrophages and neutrophils with a sub-cytotoxic concentration of BaV in vitro resulted in increased release of PGE2 and PGD2 by macrophages and PGE2 by neutrophils, concomitantly with an increment in the expression of COX-2, but not of COX-1 by both cell types. Our results demonstrate the ability of BaV to promote the expression of COX-2 and to induce the synthesis of proinflammatory prostaglandins. Macrophages and neutrophils may be important targets for this venom under in vivo situation. |
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