Fractionation of high molecular-weight duplex DNA molecules on dilute agarose gels |
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Authors: | G Henckes M Crochet B Labedan J Legault-Demare |
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Institution: | Institut du Radium-Fondation Curie, Université de Paris XI Centre d''Orsay, 91405 France |
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Abstract: | Fractionation of high molecular weight duplex DNAs was studied by electrophoresis on 0.05 to 1% agarose gels. Only the most diluted gels allowed the separation of T4 and T7 phages DNAs. The method makes use of progressive melting of the gel columns and may be applied to composite acrylamide agarose gels. |
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