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| 西洋参冠瘿组织悬浮培养及其人参皂苷类成分的分离 | |
| 引用本文： | 于荣敏,金钱星,孙辉,叶文才,赵昱.西洋参冠瘿组织悬浮培养及其人参皂苷类成分的分离[J].生物工程学报,2005,21(5):754-758. |
| 作者姓名： | 于荣敏 金钱星 孙辉 叶文才 赵昱 |
| 作者单位： | 1. 暨南大学药学院,广州,510632 2. 中国药科大学中药学院,南京,210009 3. 暨南大学药学院,广州,510632;中国药科大学中药学院,南京,210009 4. 浙江大学药学院,杭州,310031 |
| 基金项目： | 国家教育部科学技术研究重点项目基金（No.104180）,广东省自然科学基金（No.31891）,广东省中医药局科研计划项目基金（No.103041）资助.This work was supported by the grants from Ministry of Education of China （No. 104180）, Natural Sciences Foundation of Guangdong （No. 31891 ） and Chinese Traditional Medicine Administration of Guangdong, China （No. 103041 ）. |
| 摘 要： | 对西洋参冠瘿组织悬浮培养生长特征进行了考察，并对其悬浮培养物中的人参皂苷类成分进行了提取、分离和鉴定。研究得到了培养物最大生物量收获时间［18.62 g/L(dry weight)］及其中最高人参皂苷累积时间（620.4 mg/L on the 27thday）。培养基中碳源、磷、氨基氮、硝基氮的利用率分别为91.8%, 100%, 81% 和97%。利用现代分离纯化方法从培养物中分离得到了4种人参皂苷类成分，利用理化及谱学技术分别鉴定为假人参皂苷F11（pseudoginsenoside F11，Ⅰ）, 人参皂苷Rd（ginsenoside Rd，Ⅱ）, 人参皂苷Rb1（ginsenoside Rb1 ，Ⅲ）和人参皂苷Rb3（ginsenoside Rb3，Ⅳ）。 |
| 关 键 词： | 西洋参，冠瘿组织，悬浮培养，人参皂苷，分离 |
| 文章编号： | 1000-3061(2005)05-0754-05 |
| 收稿时间： | 03 25 2005 12:00AM |
| 修稿时间： | 05 25 2005 12:00AM |
The Growth Characteristics and Ginsenosides Isolation of Suspension-cultured Crown Gall of Panax quinquefolium |
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| YU Rong-Min,JIN Qian-Xing,SUN Hui,YE Wen-Cai,ZHAO Yu.The Growth Characteristics and Ginsenosides Isolation of Suspension-cultured Crown Gall of Panax quinquefolium[J].Chinese Journal of Biotechnology,2005,21(5):754-758. | |
| Authors: | YU Rong-Min JIN Qian-Xing SUN Hui YE Wen-Cai ZHAO Yu |
| Institution: | Department of Phytochemistry and Pharmacognosy, College of Pharmacy, Jinan University, Guangzhou 510632, China. rongminyu99@hotmail.com |
| Abstract: | The growth characteristics and ginsenosides isolation of the suspension-cultured crown gall of Panax quinquefolium were studied. The result showed that the maximum biomass of cultures was 18.6 g/L (dry weight) and the content of ginsenosides reached its maximum level of 620.4 mg/L on the 27th day. The utilization rates of sugar, phosphorus, nitrogen in NH4+ and nitrogen in NO3- were 91.8%, 100%, 81% and 97%, respectively. Four compounds were isolated from the suspension-cultured crown gall and their structures were elucidated as pseudoginsenoside F11 (I), ginsenoside Rd (II), ginsenoside Rb1 (llI) and ginsenoside Rb3 (IV). |
| Keywords: | crown gall ginsenosides isolation Panax quinquefolium suspension cultures |
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