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High-frequency plant regeneration via adventitious shoot formation from deembryonated cotyledon explants of Sesamum indicum L. |
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| Authors: | H. Y. Seo Y. J. Kim T. I. Park H. S. Kim S. J. Yun K. H. Park M. K. Oh M. Y. Choi C. H. Paik Y. S. Lee Y. E. Choi |
| Affiliation: | (1) Honam Agricultural Research Institute, Iksan, 570-080, South Korea;(2) International Technical Cooperation Division, Rural Development Administration, Suwon, 441-707, South Korea;(3) Chonbuk National University, Chonju, 561-756, South Korea;(4) National Institute of Crop Science, Suwon, 441-857, South Korea;(5) Division of Forest Resources, Kangwon National University, Chuncheon, 200-701, South Korea |
| Abstract: | Sesamum indicum L. was used as an important oil crop in the world. An efficient protocol for in vitro plant regeneration via adventitious shoot formation from deembryonated cotyledon explants isolated from mature seeds of sesame is developed. Optimal medium for direct adventitious shoot formation was Murashige and Skoog (MS) medium with 22.2 μM 6-benzylaminopurin (BA) and 5.7 μM indole-3-acetic acid (IAA). Abscisic acid (3.8 μM ABA) and AgNO3 (29.4 μM) were effective in enhancing the frequency of adventitious shoot formation. Preculture of cotyledon explants on high sucrose concentration (6–9%) for 2 wk and subsequent transfer to 3% sucrose enhanced the frequency of adventitious shoot induction. Root formation from the adventitious shoots was easily achieved on MS medium containing 2.7 μM of α-naphthalene acetic acid (NAA). Regenerated plantlets were acclimatized on sand and peat moss (1:1), showing 95% survival with subsequent flowering and seed set. We established the high-frequency plant regeneration via adventitious shoot formation in S. indicum L. |
| Keywords: | Sesamum indicum Adventitious shoot Plant regeneration Sesame |
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