NtTPN1: a RPP8‐like R gene required for Potato virus Y‐induced veinal necrosis in tobacco |
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| Authors: | Vincent Michel Emilie Julio Thierry Candresse Julien Cotucheau Christophe Decorps Roxane Volpatti Benoît Moury Laurent Glais François Dorlhac de Borne Véronique Decroocq Sylvie German‐Retana |
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| Institution: | 1. UMR 1332 Biologie du Fruit et Pathologie, INRA, Univ. Bordeaux, Villenave d'Ornon Cedex, France;2. Imperial Tobacco, Bergerac, France;3. Pathologie Végétale, INRA, Montfavet, France;4. FN3PT/RD3PT, Paris, France;5. IGEPP, Agrocampus Ouest, INRA, Université de Rennes 1, Le Rheu, France |
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| Abstract: | Potato virus Y (PVY) is one of the most damaging viruses of tobacco. In particular, aggressive necrotic strains (PVYN) lead to considerable losses in yield. The main source of resistance against PVY is linked to the va locus. However, va‐overcoming PVY isolates inducing necrotic symptoms were observed in several countries. In this context, it is important to find va‐independent protection strategies. In a previous study, the phenotyping of 162 tobacco varieties revealed 10 accessions that do not carry the va allele and do not exhibit typical PVYN‐induced veinal necrosis. Despite the absence of necrotic symptoms, normal viral accumulation in these plants suggests a va‐independent mechanism of tolerance to PVYN‐induced systemic veinal necrosis. Fine mapping of the genetic determinant(s) was performed in a segregating F2 population. The tolerance trait is inherited as a single recessive gene, and allelism tests demonstrated that eight of the 10 tolerant varieties carry the same determinant. Anchoring the linkage map to the tobacco genome physical map allowed the identification of a RPP8‐like R gene, called NtTPN1 (for t abacum P VY‐induced | |
| Keywords: | RPP8‐like gene
Potato virus Y
Potyvirus resistance tolerance veinal necrosis hypersensitive response R gene
Nicotiana tabacum
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