Construction and analysis of the abnormal lncRNA–miRNA–mRNA network in hypoxic pulmonary hypertension |
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| Authors: | Jie Liu Yishu Deng Zeqin Fan Shuanglan Xu Li Wei Xiaoxian Huang Xiqian Xing Jiao Yang |
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| Institution: | 1.Department of Respiratory Medicine, The Fourth Affiliated Hospital of Kunming Medical University, Kunming 650021, Yunnan, China;2.Department of Respiratory Medicine, The Affiliated Hospital of Yunnan University, The Second People’s Hospital of Yunnan Province, Kunming 650021, Yunnan, China;3.First Department of Respiratory Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China |
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| Abstract: | The incidence of hypoxic pulmonary hypertension (HPH) is increasing. Accumulating evidence suggests that long noncoding RNAs (lncRNAs) play an important role in HPH, but the functions and mechanism have yet to be fully elucidated. In the present study, we established a HPH rat model with 8 h of hypoxia exposure (10% O2) per day for 21 days. High-throughput sequencing identified 60 differentially expressed (DE) lncRNAs, 20 DE miRNAs and 695 DE mRNAs in rat lung tissue. qRT-PCR verified the accuracy of the results. The DE mRNAs were significantly enriched in immune response, inflammatory response, leukocyte migration, cell cycle, cellular response to interleukin-1, IL-17 signalling pathway, cytokine–cytokine receptor interaction and Toll-like receptor signalling pathway. According to the theory of competing endogenous RNA (ceRNA) networks, lncRNA–miRNA–mRNA network was constructed by Cytoscape software, 16 miRNAs and 144 mRNAs. The results suggested that seven DE lncRNAs (Ly6l, AABR07038849.2, AABR07069008.2, AABR07064873.1, AABR07001382.1, AABR07068161.1 and AABR07060341.2) may serve as molecular sponges of the corresponding miRNAs and play a major role in HPH. |
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| Keywords: | competing endogenous RNA differently expressed genes hypoxic pulmonary hypertension long non-coding RNAs miRNA mRNA |
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