Gas chromatographic analysis of hexosamines in glycoproteins |
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| Authors: | W Niedermeier M Tomana |
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| Affiliation: | Division of Clinical Immunology and Rheumatology, Department of Medicine, The Medical College, University of Alabama in Birmingham, Birmingham, Alabama 35294 USA |
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| Abstract: | A gas chromatographic method for the analysis of hexosamines in glycoproteins was described which uses the alditol acetate derivatives of the sugars. A polyamide (Poly A 103) liquid phase was used which effectively separates glucosamine, galactosamine, and mannosamine from each other. Mannosamine served as internal standard to facilitate accurate quantitation of glucosamine and galactosamine. |
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