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Changes in Membrane Fatty Acid Composition of Pseudomonas aeruginosa in Response to UV-C Radiations |
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| Authors: | Salma Kloula Ben Ghorbal Abdelwaheb Chatti Mohamed Marwan Sethom Lobna Maalej Mouadh Mihoub Sana Kefacha Moncef Feki Ahmed Landoulsi Abdennaceur Hassen |
| Institution: | 1. Laboratoire de Traitement des Eaux Usées, Centre des Recherches et des Technologies des Eaux (CERTE), Technopole Borj Cédria, P.O. Box 273, 8020, Soliman, Tunisia 2. Laboratoire de biochimie, H?pital La Rabta, Tunis, Tunisia 3. Unité de Biochimie des Lipides et interaction des macromolécules en Biologie, 03/UR/0902, Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Bizerte, Zarzouna, Université de Carthage, 7021, Tunis, Tunisia |
| Abstract: | The changes in lipid composition enable the micro-organisms to maintain membrane functions in the face of environmental fluctuations. The relationship between membrane fatty acid composition and UV-C stress was determined for mid-exponential phase and stationary phase Pseudomonas aeruginosa. The total lipids were obtained by dichloromethane/methanol (3:1) and were quantified by GC. The TLC analysis of phospholipids showed the presence of three major fractions phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin. Significant modifications, as manifested by an increase of UFA, were obtained. Interestingly, this microorganism showed a remarkable capacity for recovery from the stressful effects of UV-C. |
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