Molecular cloning of a novel rat gene Tsarg1, a member of the DnaJ/HSP40 protein family. |
| |
| Authors: | Hong-Mei Yang Gang Liu Zhao-Yan Nie Dong-Song Nie Yun Deng Guang-Xiu Lu |
| |
| Affiliation: | Human Reproductive and Stem Cell Engineering Institute, Central South University, Changsha, P. R. China. |
| |
| Abstract: | Beginning with a mouse gene mTSARG3, which was related to apoptosis of spermatogenic cells, bioinformatics was applied and a predicted novel rat gene full-length cDNA sequence was attained. Gene-specific primers were designed for PCR in rat testis cDNA library. A new gene Tsarg1 (GenBank Accession No. AY380804) was cloned, which is related to apoptosis in rat spermatogenic cells. The gene whose full cDNA length is 1176 bp containing 8 exons and 7 introns is located in rat chromosome 1q32-1q33, which encoded a protein containing 316 amino acid residues and being a new member of HSP40 protein family since the sequence contains the highly conserved J domain, which is present in all DnaJ-like proteins and is supported to have a critical role in DnaJ-DnaK protein-protein interactions. The results of RT-PCR and Northern blot analysis showed that Tsarg1 was specifically expressed in rat testis, which probably inhibits rat testis spermatogenic cell apoptosis. |
| |
| Keywords: | |
|
|
