Autofluorescence of the fungus <Emphasis Type="Italic">Morchella conica</Emphasis> var. <Emphasis Type="Italic">rigida</Emphasis> |
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Authors: | Z ?i?ka J Gabriel |
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Institution: | (1) Institute of Microbiology of the ASCR, v.v.i., 142 20, Praha 4, Czech Republic |
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Abstract: | Autofluorescence (primary fluorescence (AF)) of fruiting bodies and stems of the fungus Morchella conica var. rigida was studied by fluorescence microscopy including sporangia and ascospores. The ascospores were characterized by a weak green–yellow
AF at blue excitation. Using a green excitation, no AF was observed. The hyphae located under the layer of asci with ascospores
exhibited a higher primary fluorescence, namely their walls that had green-yellow color at blue excitation. Also, their red
AF observed when a green excitation was used was significant. Similarly, the hyphae located in the fungal stem exhibited a
significant AF, especially their walls when the blue light was used for excitation. In addition, large, yellow-to-yellow/green,
oval-to-round bodies with strong fluorescence were detected whose morphological equivalents were not clearly visible in the
white halogen light. The AF of the fungus M. conica var. rigida was lower compared with the other higher fungi studied so far. |
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Keywords: | |
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