Characterization of pyruvate decarboxylase genes from rice |
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Authors: | M Anwar Hossain Enamul Huq Anil Grover Elizabeth S Dennis W James Peacock Thomas K Hodges |
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Institution: | (1) Department of Botany & Plant Pathology, Purdue University, 47907 West Lafayette, IN, USA;(2) CSIRO Division of Plant Industry, GPO Box 1600, 2601 Canberra, Act, Australia;(3) Present address: Department of Biochemistry, University of Dhaka, 1000 Dhaka, Bangladesh;(4) Present address: Department of Plant Molecular Biology, University of Delhi, South Campus, Benito Juarez Road, 110021 New Delhi, India |
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Abstract: | The pdc1 gene encoding pyruvate decarboxylase has been isolated and sequenced from an IR54 rice genomic library. In contrast to a previously isolated intron-less rice genomic pdc, pRgpdc3, this gene contains five intervening introns in the coding region and corresponds to a cDNA clone, pRcpdc1, isolated from an IR54-cDNA library constructed from anaerobically-induced mRNAs. Comparison of the deduced amino acid sequence of this gene with that of the rice pdc2 and pdc3 showed 88% and 89% similarity, and 78% and 79% identity, respectively. Southern blots indicated that more than three genes constitute the pdc gene family in rice. pdc1 is highly inducible under anaerobic conditions. Rice pdc2 is also inducible by anoxia but to a much lesser extent than pdc1. |
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Keywords: | anoxia pyruvate decarboxylase rice submergence tolerance |
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