Taxonomic diversity of aerobic organotrophic bacteria from clean vietnamese soils and their capacity for oxidation of petroleum hydrocarbons |
| |
Authors: | Email author" target="_blank">A?E?IvanovaEmail author I?A?Borzenkov E?A?Strelkova Nguen?Thu?Hoai Do?Ngok?Lan S?S?Belyaev V?A?Karpov |
| |
Institution: | 1.Winogradsky Institute of Microbiology,Russian Academy of Sciences,Moscow,Russia;2.Joint Russian-Vietnamese Tropical Research and Technological Center,Hanoi,Vietnam;3.Severtsov Institute of Ecology and Evolution,Russian Academy of Sciences,Moscow,Russia |
| |
Abstract: | The dominant species and abundance of the cultured aerobic organotrophic bacteria were determined in the clean soils of the
Republic of Vietnam. The total number of organotrophs varied from 2.0 × 105 to 5.8 × 108 CFU/g soil. A considerable fraction of the bacterial population (1.1 × 105–9.5 × 106 CFU/g soil) was able to utilize petroleum hydrocarbons as the sole carbon and energy source. Most of the organisms obtained
in pure cultures were gram-positive bacteria; over 70% were hydrocarbon-oxidizing organisms. Analysis of 16S rRNA gene sequences
resulted in tentative determination of the taxonomic position of 22 strains, with 12 belonging to the Firmicutes, 4, to the Proteobacteria, and 6 to the Actinobacteria. The most common bacteria capable of hydrocarbon oxidation belonged to the genera Acinetobacter, Bacillus, Brevibacillus, Chromobacterium, Cupriavidus, Gordonia, Microbacterium, Mycobacterium, and Rhodococcus. Some of the isolated Bacillus and Staphylococcus strains, as well as one Pseudomonas and one Sinomonas strain, did not utilize hydrocarbons. Gram-positive degraders, especially members of the order Actinomycetales, which exhibited high hydrocarbon-oxidizing activity, gained competitive advantage in the presence of hydrocarbons. This
microbial group probably plays an important role in hydrocarbon degradation in tropical soils. Thus, Vietnamese soils, which
had no history of petroleum contamination, support numerically significant and taxonomically diverse populations of h ydrocarbon-oxidizing
bacteria. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|