Evaluation of the V404I and V509M amino acid substitutions of <Emphasis Type="Italic">ERG11</Emphasis> gene in <Emphasis Type="Italic">Candida albicans</Emphasis> isolates by pyrosequencing |
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| Authors: | T -H Kim M -K Lee |
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| Institution: | (1) Department of Dermatology, Tianjin Medical University General Hospital, Tianjin Medical University, Tianjin, PR China;(2) Centre for Infectious Diseases and Microbiology, The University of Sydney, Westmead Hospital, Sydney, Australia;(3) Retroviral Genetics Laboratory, Centre for Virus Research, Westmead Millennium Institute and the University of Sydney, Sydney, Australia;(4) Schering-Plough Research Institute, Kenilworth, New Jersey, USA;(5) Mycology Unit, Women's and Children's Hospital, Adelaide, Australia;(6) Life Science College, Peking University, Beijing, PR China |
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| Abstract: | The molecular mechanisms underlying fluconazole resistance in C. albicans involve mutations and the overexpression of the ERG11 gene and membrane transport proteins. We examined the relationship between the reduced fluconazole susceptibility of C. albicans and mutations of V404I and V509M in the ERG11 gene in 182 C. albicans clinical isolates using the Pyrosequencing™ method. DNAs from these clinical isolates with different levels of in-vitro fluconazole susceptibility — one resistant, five susceptible dose-dependent (SDD), four trailer, and 172 susceptible — were
analyzed. None of the fluconazole-susceptible, SDD, trailer or resistant isolates had mutations of V404I or V509M. Our results
showed that no correlation can be found between the V404I or V509M mutation and fluconazole susceptibility in C. albicans. |
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