C群奈瑟氏脑膜炎球菌荚膜多糖-TT结合疫苗的制备及其在小鼠体内免疫原性的研究

将C群脑膜炎球菌荚膜多糖以ADH作为间隔剂与TT结合形成GCMP-TT结合疫苗,然后用此结合疫苗免疫NIH小鼠,结果显示使用GCMP免疫小鼠后仅能产生较低水平抗GCMP的IgG抗体,而用GCMP-TT免疫后小鼠血清中产生了较GCMP免疫显著增高抗GCMP的IgG抗体,并且GCMP-TT组第二次和第三次免疫后与初次免疫相比,IgG抗体水平均有显著升高(P<0.01),表明GCMP-TT结合疫苗具有免疫记忆和再次免疫加强应答效应。补体介导的血清抗体体外杀菌试验结果证明,GCMP-TT结合疫苗组免疫小鼠诱导的抗体IgG比GCMP组具有增强的体外杀菌活性。

Preparation,characterization and immunogenicity in mice of Neisseria meningococcal serogroup C capsular poly-saccharide-tetanus toxoid conjugate vaccines

ADH as the spacer, meningococcal capsular polysaccharide of serogroup C was conjugated with tetanus toxoid(TT). It showed that anti-GCMP IgG antibodies could not be elicited at a higher level in the mice immunized by GCMP alone,whereas IgG antibodies of both anti-GCMP and anti-TT immunized by GCMP-TT conjugates in the mice could be elicited at a higher level. The anti-GCMP IgG antibodies after the second and third immunization were remarkablely higher than those after the first immunization (P<0.01). The results also showed that immunological memory and booster responses could be elicited and enhanced by GCMP-TT conjugates. The GCMP IgG antibodies had a stronger bactericidal activity elicited in mice by GCMP-TT conjugates than that by GCMP by means of the complement- dependent bactericidal experiment in vitro.