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| 微生物发酵炮制何首乌机理的初步研究 | |
| 引用本文： | 杜晨晖,海青山,闫艳,原红霞,赵荣华,赵声兰,王兴红.微生物发酵炮制何首乌机理的初步研究[J].天然产物研究与开发,2012,24(2):212-215. |
| 作者姓名： | 杜晨晖 海青山 闫艳 原红霞 赵荣华 赵声兰 王兴红 |
| 作者单位： | 1. 山西中医学院,太原,030024 2. 云南中医学院中药学院,昆明,6505002 3. 云南大学云南省微生物研究所,昆明,650091 |
| 基金项目： | 国家自然科学基金项目(30760312) |
| 摘 要： | 为探讨微生物发酵炮制何首乌的机制,采用HPLC考察何首乌微生物发酵前后二苯乙烯苷类和蒽醌类化学成分的变化。何首乌经米根霉发酵后,产生了新的蒽醌类成分大黄素-6-O-β-D-吡喃葡萄糖苷,而二苯乙烯苷类成分无变化。同时药理研究发现,大黄素-6-O-β-D-吡喃葡萄糖苷对家兔肠平滑肌的收缩作用弱于大黄素。由此推断,在何首乌发酵炮制过程中,米根霉可催化大黄素转化为大黄素-6-O-β-D-吡喃葡萄糖苷,从而降低何首乌的泻下作用。实验结果初步验证了微生物发酵炮制何首乌的科学性。 |
| 关 键 词： | 微生物发酵 何首乌 大黄素 大黄素-6-O-β-D-吡喃葡萄糖苷 兔离体回肠 |
Preliminary Study on the Processing Mechanism of Polygonum multiflorum Thunb by Microbial Fermentation |
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| DU Chen-hui , HAI Qing-shan , YAN Yan , YUAN Hong-xia , ZHAO Rong-hua , ZHAO Sheng-lan , WANG Xing-hong.Preliminary Study on the Processing Mechanism of Polygonum multiflorum Thunb by Microbial Fermentation[J].Natural Product Research and Development,2012,24(2):212-215. | |
| Authors: | DU Chen-hui HAI Qing-shan YAN Yan YUAN Hong-xia ZHAO Rong-hua ZHAO Sheng-lan WANG Xing-hong |
| Institution: | 1Shanxi College of Traditional Chinese Medicine,Taiyuan 030024,China;2College of Traditional Chinese Pharmacy,Yunnan College of Traditional Chinese Medicine,Kunming 650500,China;3Yunnan University,Microbe Research Institute,Kunming 650091,China |
| Abstract: | The aim of this study was to investigate the processing mechanism of Polygonum multiflorum Thunb by microbial fermentation.The anthraquinones and di-phenyl ethylene glycoside in fermented and no-fermented samples were determined by HPLC, and emodin-6-O-β-D-glucopyranoside was produced in P.multiflorum after fermentation.However,no evident change was identified for di-phenyl ethylene glycoside.In addition,the contraction action of the emodin-6-O-β-D-glucopyranoside was weaker than that of emodin on excised rabbit ileum,which suggested that the purgative activity of P.multiflorum was reduced after fermentation.The results preliminarily indicate that the processing for P.multiflorum by microbial fermentation is scientific. |
| Keywords: | microorganism fermentation Polygonum multiflorum Thunb emodin emodin-6-O-β-D-glucopyranoside excised rabbit ileum |
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