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A study on the mechanism of the vanadate-dependent NADH oxidation |
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| Authors: | Stefan I Liochev and Ekaterina A Ivancheva |
| Institution: | Department of Bioenergetics, Institute of Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev St., bl. 23, 1113, Sofia, Bulgaria |
| Abstract: | The mechanism of the vanadate (V(v))-dependent oxidation of NADH was different in phosphate buffers and in phosphate-free media. In phosphate-free media (aqueous medium or HEPES buffer) the vanadyl (V(v)) generated by the direct V(v)-dependent oxidation of NADH formed a complex with V(v). In phosphate buffers V(v) autoxidized instead of forming a complex with V(v). The generated superoxide radical (O2−) initiated, in turn, a high-rate free radical chain oxidation of NADH. Phosphate did not stimulate the V(v)-dependent NADH oxidation catalyzed by O2−-generating systems. Monovanadate proved to be a stronger catalyzer of NADH oxidation as compared to polyvanadate. |
| Keywords: | Vanadate Vanadyl Superoxide radical NADH SOD Vanadate-dependent NADH oxidation Free radical |
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