Isolation of hyaluronic acid by gel filtration on agarose |
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| Affiliation: | 2. School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, United States;3. Department of Micro- and Nanotechnology, Center for Nanomedicine and Theranostics, Technical University of Denmark, Kgs. Lyngby, Denmark;4. Center for Biomedical Research, University of Victoria, Victoria, Canada;5. Center for Advanced Materials and Related Technologies, University of Victoria, Victoria, Canada |
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| Abstract: | A method is described for preparing an undegraded, hyaluronic acid-protein complex from synovial fluid, and which gives, at the same time, a picture of molecular weight heterogeneity in the hyaluronic acid. The protein is shown to be firmly bound to the polysaccharide. |
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