Isolation of hyaluronic acid from human synovial fluid by pronase digestion and gel filtration |
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| Affiliation: | 1. Anesthesia and Critical Care Department, Hamedan University of Medical Sciences, Hamedan, Iran;2. Trauma Research Center, Nursing Faculty, Baqiyatallah University of Medical Sciences, Tehran, Iran;3. Anesthesiology Research Center, Anesthesia and Critical Care Department, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran;4. Research Center for Prevention of Oral and Dental Disease, Baqiyatallah University of Medical Sciences, Tehran, Iran;5. Tracheal Diseases Research Center, Anesthesia and Critical Care Department, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran;6. Trauma Research Center, Medicine Faculty, Baqiyatallah University of Medical Sciences, Tehran, Iran;7. Department of Internal Medicine, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran;8. Anesthesia and Critical Care Department, Alborz University of Medical Sciences, Karaj, Iran;9. Anesthesiology Research Center, Anesthesia Care Department, Modares Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran;10. Department of Medicine, Division of Pulmonary, Critical Care Medicine and Sleep, University of South Florida, Tampa, FL, USA;11. Department of Medicine, Division of Pulmonary, Critical Care Medicine and Sleep, James A. Haley Veteran Hospital, Tampa, FL, USA;12. Department of Emergency Medicine, J.W. Ruby Memorial Hospital, West Virginia University, Morgantown, WV, USA;13. Department of Emergency Medicine, Vident Medical Center, East Carolina University, Greenville, NC, USA |
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| Abstract: | The isolation of hyaluronic acid from human synovial fluid by passage down Sephadex G-200, following sterile digestion with pronase in Tris buffer (pH 7.9) for 8 h at 37°, affords a product without the occurrence of the enzymic or chemical degradation that may accompany other methods of isolation. Use of phosphate buffer, under similar conditions, gives turbid solutions of hyaluronic acid. |
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