Initiation and characterization of a diploid cell line from larval tissues ofAedes dorsalis (Meigen) |
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| Authors: | Barbara E. Cahoon James L. Hardy William C. Reeves |
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| Affiliation: | (1) Department of Biomedical and Environmental Health Sciences, University of California, 74720 Berkeley, California;(2) Naval Biosciences Laboratory, School of Public Health, University of California, 74720 Berkeley, California;(3) School of Public Health, Warren Hall, University of California, 94720 Berkeley, California |
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| Abstract: | Summary Mosquito cell cultures were initiated from the minced tissues of newly hatchedAedes dorsalis (Meigen) larvae. Continuous cell division occurred only after an adaptive period of approximately 6 months. Optimal growth of the cells required a relatively low pH of 6.5. Karyological studies showed that the cells have remained diploid (2n=6) for 60 serial passages and that the cultures are free of contaminating cells. The cultures also were shown to be free of bacteria (includingMycoplasma), fungi and virions. Subpopulations (strains) of the original parental cultures have been selected and characterized on the basis of morphology, karyology, growth rate and monolayer formation. These studies were supported in part by funds from the Office of Naval Research, by Research Grant AI03028 from the National Institute of Allergy and Infectious Diseases, and by General Research Support Grant I-SO1-FR-05441 from the National Institutes of Health, U.S. Department of Health, Education and Welfare. |
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| Keywords: | Aedes mosquito cell line diploid |
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