卫星RNA对黄瓜花叶病毒基因组RNA体外合成的影响

卫星ＲＮＡ对黄瓜花叶病毒基因组ＲＮＡ体外合成的影响杨海花，康良仪，赵大健，田波（中国科学院微生物研究所，北京１０００８０）关键词卫星ＲＮＡ，黄瓜花叶病毒，依赖ＲＮＡ的ＲＮＡ聚合酶，体外合成利用卫星ＲＮＡ生防制剂控制田间的番茄、青椒、烟草等由黄瓜花叶病...

The Effect of Satellite RNAs on Replication of Cucumber Mosaic Virus Genomic RNAs in vitro

NA - dependent RNA polymerase extracted from the cucumber cotyledons four days afterinoculation by CMV-B-1 containing attenuation satellite RNA was used to initiate synthesis of(-)sense RNA from( )sense RNA,using both CMV genomic or satellite RNAs as tem-plates.In presence of satellite RNA,the synthesis of CMV genomic RNA1,RNA2 and RNA4was inhibited.However,different satellite RNAs showed different effects on the synthesis of viralRNAs.Adisease-attenuated satellite RNA-1 decreased the synthe.is of CMV RNA4,but thetomato necrosis-induced satellite RNA-D increased the synthesis of CMV RNA4.The resultindicated that the satellite RNAs could interfere with synthesis of CMV coat protein in the CMV-infected tissues and modulate plant symptoms by regulating the synthesis of CMV RNA1,RNA2 and RNA4.