| 热休克因子1通过上调蛋白质C减轻脓毒症凝血功能障碍保护小鼠急性肺损伤 |
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| 引用本文： | 王浩,李涛,肖归,刘梅冬,刘可,张华莉,朱雅茜,肖献忠.热休克因子1通过上调蛋白质C减轻脓毒症凝血功能障碍保护小鼠急性肺损伤[J].生物化学与生物物理进展,2022,49(4):788-797. |
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| 作者姓名： | 王浩 李涛 肖归 刘梅冬 刘可 张华莉 朱雅茜 肖献忠 |
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| 作者单位： | 1）中南大学基础医学院病理生理学系，长沙 410008;2）脓毒症转化医学湖南省重点实验室，长沙 410008;3）医学机能学国家级实验教学中心，长沙 410078,4）嘉应学院医学院病理生理学教研室，梅州 514031,5）中南大学湘雅护理学院，长沙 410008,1）中南大学基础医学院病理生理学系，长沙 410008;2）脓毒症转化医学湖南省重点实验室，长沙 410008;3）医学机能学国家级实验教学中心，长沙 410078,1）中南大学基础医学院病理生理学系，长沙 410008;2）脓毒症转化医学湖南省重点实验室，长沙 410008;3）医学机能学国家级实验教学中心，长沙 410078,1）中南大学基础医学院病理生理学系，长沙 410008;2）脓毒症转化医学湖南省重点实验室，长沙 410008;3）医学机能学国家级实验教学中心，长沙 410078,1）中南大学基础医学院病理生理学系，长沙 410008;2）脓毒症转化医学湖南省重点实验室，长沙 410008;3）医学机能学国家级实验教学中心，长沙 410078,1）中南大学基础医学院病理生理学系，长沙 410008;2）脓毒症转化医学湖南省重点实验室，长沙 410008;3）医学机能学国家级实验教学中心，长沙 410078 |
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| 基金项目： | 国家自然科学基金（81871610，82172146，82000742）资助项目。 |
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| 摘 要： | 目的 探讨热休克因子1 （HSF1）减轻脓毒症凝血功能障碍，保护小鼠急性肺损伤的机制。方法 本研究采用盲肠结扎穿孔术（cecal ligation and puncture,CLP）制备脓毒症小鼠模型，检测凝血相关指标和观察小鼠肺部病理变化，通过酶联免疫吸附实验（ELISA）、q RT-PCR和蛋白质印迹法（Western blot）等方法检测蛋白质C表达水平，通过质粒转染抑制或增强HSF1表达从而观察蛋白质C表达水平的变化，并利用生物信息学、凝胶电泳迁移实验（EMSA）和双荧光素酶报告基因实验探讨HSF1调节蛋白质C转录的机制。结果 在CLP脓毒症小鼠模型中，HSF-/-组小鼠的凝血活性与HSF1+/+组相比明显增强，肺损伤明显加重。ELISA、qRT-PCR和Western blot检测发现，HSF-/-脓毒症小鼠血浆和肺组织中的蛋白质C表达水平显著低于野生型小鼠。体外bEnd.3血管内皮细胞的实验结果显示，HSF1抑制脂多糖（LPS）诱导的蛋白质C表达，HSF1过表达则增强蛋白质C表达。生物信息学数据分析提示，蛋白质C启动子区含有HSF...
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| 关 键 词： | 热休克因子1 脓毒症 蛋白质C 凝血 急性肺损伤 |
| 收稿时间： | 2022/3/15 0:00:00 |
| 修稿时间： | 2022/3/22 0:00:00 |
HSF1 Attenuates Coagulatory Dysfunction and Protects Mice From Acute Lung Injury by Upregulating Protein C in Sepsis |
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| WANG Hao,LI Tao,XIAO Gui,LIU Mei-Dong,LIU Ke,ZHANG Hua-Li,ZHU Ya-Xi and XIAO Xian-Zhong.HSF1 Attenuates Coagulatory Dysfunction and Protects Mice From Acute Lung Injury by Upregulating Protein C in Sepsis[J].Progress In Biochemistry and Biophysics,2022,49(4):788-797. |
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| Authors: | WANG Hao LI Tao XIAO Gui LIU Mei-Dong LIU Ke ZHANG Hua-Li ZHU Ya-Xi and XIAO Xian-Zhong |
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| Institution: | 1)Department of Pathophysiology, School of Basic Medicine Science, Central South University, Changsha 410008, China;2)Sepsis Translational Medicine Key Laboratory of Hunan Province, Central South University, Changsha 410008, China;3)National Medicine Functional Experimental Teaching Center, Central South University, Changsha 410078, China,4)Department of Pathophysiology, Medicine College of Jiaying University, Meizhou 514031, China,5)Xiangya School of Nursing, Central South University, Changsha 410008, China,1)Department of Pathophysiology, School of Basic Medicine Science, Central South University, Changsha 410008, China;2)Sepsis Translational Medicine Key Laboratory of Hunan Province, Central South University, Changsha 410008, China;3)National Medicine Functional Experimental Teaching Center, Central South University, Changsha 410078, China,1)Department of Pathophysiology, School of Basic Medicine Science, Central South University, Changsha 410008, China;2)Sepsis Translational Medicine Key Laboratory of Hunan Province, Central South University, Changsha 410008, China;3)National Medicine Functional Experimental Teaching Center, Central South University, Changsha 410078, China,1)Department of Pathophysiology, School of Basic Medicine Science, Central South University, Changsha 410008, China;2)Sepsis Translational Medicine Key Laboratory of Hunan Province, Central South University, Changsha 410008, China;3)National Medicine Functional Experimental Teaching Center, Central South University, Changsha 410078, China,1)Department of Pathophysiology, School of Basic Medicine Science, Central South University, Changsha 410008, China;2)Sepsis Translational Medicine Key Laboratory of Hunan Province, Central South University, Changsha 410008, China;3)National Medicine Functional Experimental Teaching Center, Central South University, Changsha 410078, China,1)Department of Pathophysiology, School of Basic Medicine Science, Central South University, Changsha 410008, China;2)Sepsis Translational Medicine Key Laboratory of Hunan Province, Central South University, Changsha 410008, China;3)National Medicine Functional Experimental Teaching Center, Central South University, Changsha 410078, China |
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| Abstract: | |
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| Keywords: | HSF1 sepsis protein C coagulation acute lung injury |
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