Development of a Biochip for Analyzing Polymorphism of the Biotransformation Genes

Large-scale population studies, diagnosis of genetic predisposition to a broad range of multifactorial diseases, and screening of polymorphic loci associated with individual drug resistance need efficient, accurate, and rapid techniques for identifying many mutations. One of the most promising techniques is hybridization on an oligonucleotide microarray (biochip). The efficiency of this method in assessing genetic polymorphism was demonstrated using an example of mutations in CYP1A1, CYP2D6, GSTM1, GSTT1, NAT2, CYP2C9, CYP2C19, and MTHFR. The biochip constructed provides a convenient tool for pharmacogenetic research.__________Translated from Molekulyarnaya Biologiya, Vol. 39, No. 3, 2005, pp. 403–412.Original Russian Text Copyright © 2005 by Glotov, Nasedkina, Ivaschenko, Urasov, Surzhikov, Pan’kov, Chudinov, Baranov, Zasedatelev.