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| 骨髓动员对骨髓造血干细胞在心肌梗死后心脏中定植及分化的影响 | |
| 引用本文： | 田文,曾定尹,Sigrid Nikol. 骨髓动员对骨髓造血干细胞在心肌梗死后心脏中定植及分化的影响[J]. 中国组织化学与细胞化学杂志, 2009, 18(3): 304-310. DOI: 10.3870/zgzzhx.2009.03.014 |
| 作者姓名： | 田文 曾定尹 Sigrid Nikol |
| 作者单位： | 1. 中国医科大学附属第一医院心内科,沈阳,110001 2. 德国明斯特大学医院心血管内科,D-48149 |
| 摘 要： | 目的观察小鼠心肌梗死后骨髓造血干细胞在心脏内的分化及细胞因子的影响。方法C57/BL6小鼠60只分为骨髓动员组和对照组,先后行脾切除、骨髓移植（骨髓供体为增强绿色荧光蛋白转基因小鼠）、骨髓动员及建立心肌梗死模型。心肌梗死后3周将小鼠心脏取出并切片行组织学及激光共聚焦显微镜免疫荧光检查。结果骨髓动员可以增加EGFP阳性细胞在心脏中梗死区和边缘区的定植,但绝大多数EGFP阳性细胞都同时表达CD45。仅发现有极少数骨髓来源的心肌细胞、成纤维细胞及血管内皮细胞,且与骨髓动员无相关性。结论骨髓动员能够明显促进骨髓来源细胞定植入小鼠心脏的梗死区;极少数骨髓造血干细胞可以分化为心肌细胞,其数量远不足以修复梗死心肌及改善心功能;骨髓造血干细胞不参与梗死区疤痕形成的病理过程。 |
| 关 键 词： | 骨髓造血干细胞 骨髓动员 增强绿色荧光蛋白 共聚焦激光扫描 心肌梗死 |
INFLUENCE OF BONE MARROW MOBILIZATION ON BONE MARROW HEMATOPOIETIC STEM CELLS' ENGRAFTMENT AND DIFFERENTIATION IN MOUSE INFARCTED HEART |
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| Tian Wen,Zeng Dingyin,Michael Kuhlmann,Sigrid Nikol. INFLUENCE OF BONE MARROW MOBILIZATION ON BONE MARROW HEMATOPOIETIC STEM CELLS' ENGRAFTMENT AND DIFFERENTIATION IN MOUSE INFARCTED HEART[J]. Chinese Journal of Histochemistry and Cytochemistry, 2009, 18(3): 304-310. DOI: 10.3870/zgzzhx.2009.03.014 | |
| Authors: | Tian Wen Zeng Dingyin Michael Kuhlmann Sigrid Nikol |
| Affiliation: | Tian Wen1,Zeng Dingyin1,Michael Kuhlmann2,Sigrid Nikol2(1Department of Cardiology,The First Affiliated Hospital of China Medical University,Shenyang,110001,China,2Department of Cardiology and Angiology,The Hospital of Munster University,Munster D-48149,Germany) |
| Abstract: | Objective To observe the differentiation of bone marrow hematopoietic stem cells(BMHSCs) in murine infarcted heart and identify the cytokines' influence on the differentiation.Methods Sixty C57/BL6 mice were randomly grouped and treated with splenectomy and bone marrow transplantation from EGFP transgenic donor.Bone marrow was mobilized by granulocyte clone stimulating factor(G-CSF) and stem cell factor(SCF) and myocardial infarction was induced successively.The infarcted hearts were taken out and sectioned... |
| Keywords: | Bone marrow hematopoietic stem cell(BMHSC) Bone marrow mobilization EGFP Confocal laser scanning Myocardial infarction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |