Population genetic structure of <Emphasis Type="Italic">Sargassum thunbergii</Emphasis> (Fucales,Phaeophyta) detected by RAPD and ISSR markers |
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Authors: | Fengjuan Zhao Xiuliang Wang Jidong Liu Delin Duan |
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Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China;(2) Graduate School of Chinese Academy of Sciences, Beijing, 100039, China |
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Abstract: | Genetic variation of four populations of Sargassum thunbergii (Mert.) O. Kuntze and one outgroup of S. fusiforme (Harv.) Setchell from Shandong peninsula of China was studied with random amplified polymorphic DNA (RAPD) and inter-simple
sequence repeat (ISSR) markers. A total of 28 RAPD primers and 19 ISSR primers were amplified, showing 174 loci and 125 loci,
respectively. Calculation of genetic diversity with different indicators (P%, percentage of polymorphic loci; H, the expected heterozygosity; I, Shannon’s information index) revealed low or moderate levels of genetic variations within each S. thunbergii population. High genetic differentiations were determined with pairwise Nei’s unbiased genetic distance (D) and fixation index (F
ST
) between the populations. The Mantel test showed that two types of matrices of D and F
ST
were highly correlated, whether from RAPD or ISSR data, r = 0.9310 (P = 0.008) and 0.9313 (P = 0.009) respectively. Analysis of molecular variance (AMOVA) was used to apportion the variations between and within the
S. thunbergii populations. It indicated that the variations among populations were higher than those within populations, being 57.57% versus
42.43% by RAPD and 59.52% versus 40.08% by ISSR, respectively. Furthermore, the Mantel test suggested that the genetic differentiations
between the four populations were related to the geographical distances (r > 0.5), i.e., they conformed to the IBD (isolation by distance) model, as expected from UPGMA (unweighted pair group method
with arithmetic averages) cluster analysis. As a whole, the high genetic structuring between the four S. thunbergii populations along distant locations was clearly indicated in the RAPD and ISSR analyses (r > 0.8) in our study. |
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Keywords: | genetic structure genetic variability RAPD ISSR Sargassum thunbergii Phaeophyta |
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