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Non-cytotoxic silver nanoparticle-polyvinyl alcohol hydrogels with anti-biofilm activity: designed as coatings for endotracheal tube materials |
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| Authors: | Ching-Yee Loo Paul M. Young Wing-Hin Lee Rosalia Cavaliere Cynthia B. Whitchurch |
| Affiliation: | 1. Advanced Drug Delivery Group, Faculty of Pharmacy, University of Sydney, Sydney, NSW 2006, Australia;2. Woolcock Institute of Medical Research, University of Sydney, Glebe, NSW 2037, Australia;3. Woolcock Institute of Medical Research, University of Sydney, Glebe, NSW 2037, Australia;4. Discipline of Pharmacology, Sydney Medical School, University of Sydney, NSW 2050, Australia;5. The ithree Institute, University of Technology Sydney, Ultimo, NSW 2007, Australia |
| Abstract: | Endotracheal intubation is commonly associated with hospital-acquired infections as the intubation device acts as reservoir for bacterial colonization in the lungs. To reduce the incidence of bacterial colonization on the tubes, hydrogel coatings loaded with antimicrobial agents are gaining popularity. The aim of this study was to incorporate silver nanoparticles (AgNPs) into polyvinyl alcohol (PVA) to form stable hydrogels. Embedding AgNPs into PVA resulted in a decreased elongation at break and an increased tensile strength compared to PVA alone. The Ag release profile varied as a function of the degree of hydrolysis of PVA: the higher degree of hydrolysis demonstrated a lower release rate. Fourier infrared transform spectroscopy demonstrated that AgNPs interacted exclusively with the –OH groups of PVA. AgNP-loaded PVA was non-toxic against human normal bronchial epithelial cells while effective against the attachment of Pseudomonas aeruginosa and Staphylococcus aureus with a greater effect on P. aeruginosa. |
| Keywords: | silver nanoparticles PVA endotracheal intubation biofilm endotracheal tube antimicrobial |