| Reverse ChIP:研究DNA-蛋白质相互作用的新方法 |
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| 引用本文： | 赵明明,齐锦生,栗彦宁.Reverse ChIP:研究DNA-蛋白质相互作用的新方法[J].中国生物化学与分子生物学报,2010,26(5):407-410. |
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| 作者姓名： | 赵明明 齐锦生 栗彦宁 |
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| 作者单位： | 河北医科大学生物化学与分子生物学教研室，石家庄050017；河北医科大学实验动物学部分子生物学研究室，石家庄050017 |
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| 基金项目： | 河北省自然科学基金资助项目(No.C2009001092); 河北省科技厅科技攻关计划(No.07276101D-73); 石家庄市科技局科技攻关计划(No.07120803A)~~ |
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| 摘 要： | 反向染色质免疫共沉淀技术(reverse chromatin immunoprecipitation assay,Reverse ChIP)是一种在体内状态下分析DNA-蛋白质相互作用的新方法.它用特异的核酸探针捕获靶DNA片段及与其相结合的蛋白质,蛋白质用质谱仪检测,以达到确定靶DNA位点全部相关蛋白质的目的.其可对靶DNA位点相关蛋白质进行全面、系统地鉴定,特别是寻找已知DNA元件相应的调节蛋白.在发现、鉴定靶DNA位点相关蛋白质和研究DNA-蛋白质相互作用中有重要应用价值.
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| 关 键 词： | 反向染色质免疫共沉淀技术(Reverse ChIP) DNA-蛋白质相互作用 靶DNA位点相关蛋白质 |
| 收稿时间： | 2009-12-21 |
Reverse ChIP:A New Method to Study DNA-Protein Interactions |
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| ZHAO Ming-Ming,QI Jin-Sheng,LI Yan-Ning.Reverse ChIP:A New Method to Study DNA-Protein Interactions[J].Chinese Journal of Biochemistry and Molecular Biology,2010,26(5):407-410. |
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| Authors: | ZHAO Ming-Ming QI Jin-Sheng LI Yan-Ning |
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| Institution: | DepartmentofBiochemistryandMolecularBiology,HebeiMedicalUniversity,Shijiazhuang050017,China;DepartmentofMolecularBiology,HebeiKeyLaboratoryofLaboratoryAnimal,HebeiMedicalUniversity,Shijiazhuang050017,China |
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| Abstract: | Reverse chromatin immunoprecipitation assay (reverse ChIP) is a new method to study DNA-protein interaction in vivo. It uses a specific nucleic acid probe to isolate genomic DNA with its associated proteins and identifies all the proteins bound to those loci using mass spectrometric analysis. This method allows the unbiased identification of targeted DNA locus-associated proteins, especially to find the regulatory proteins associated with the known DNA elements. Reverse ChIP will be of great value in the identification of targeted DNA locus-associated proteins and the study of DNA-protein interaction. |
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| Keywords: | reverse chromatin immunoprecipitation assay(reverse ChIP) DNA-protein interactions targeted DNA locus-associated proteins |
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