The synthesis of ribosomal protein and ribosomal RNA in a rat-mouse hybrid cell line

A rat-mouse hybrid cell line was examined for the presence of ribosomal RNA and ribosomal proteins from both parents. As demonstrated by banding of centromeric heterochromatin, the hybrid cell line contained most of the mouse genome and at least 13 rat chromosomes. The ability of rat, but not mouse, ribosomes to dimerize was used to show that the hybrid contained both rat and mouse 28S ribosomal RNA. Two-dimensional polyacrylamide gel electrophoresis of ribosomal proteins indicated the presence of both rat and mouse ribosomal proteins.