Inhibition of movement and ATP-ase activity of demembranated sea urchin spermatozoa by anti-dynein antiserum.

Anti-dynein antiserum was prepared in rabbits against a tryptic fragment (Fragment A) of dynein ATPase. The antiserum inhibited the ATP-induced movement of demembranated sea urchin spermatozoa. The complete inhibition of progressive movement occurred at relatively low concentrations of the antiserum, which affected the ATPase activity to a rather small extent. The anti-dynein antiserum seems to inhibit not only ATPase activity but also bending-wave formation, in some way.