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Synthesis of biotinylated muramyl tripeptides with NOD2-stimulating activity |
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| Authors: | Gisch Nicolas Buske Birte Heine Holger Lindner Buko Zähringer Ulrich |
| Institution: | a Department of Molecular Infection Biology, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 1-40, 23845 Borstel, Germany b Department of Immunology and Cell Biology, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 1-40, 23845 Borstel, Germany |
| Abstract: | Muramyl di- and tri-peptides are putative activators of the innate immune system through stimulation of the NOD2 receptor. To provide tools for the clarification of the mechanism of this activation we isolated different UDP-muramyl tripeptides (Lys- and DAP-type) from bacteria and used them to synthesize biotinylated derivatives. All biotinylated compounds retained their ability to activate NOD2 in a cell-based test system and are therefore suitable for binding studies aimed at identifying the appropriate pattern recognition receptor(s). |
| Keywords: | Glycopeptides Biotinylation Peptidoglycan NOD2 |
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