Osteogenic activity of diphenyl ether-type cyclic diarylheptanoids derived from Acer nikoense |
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Authors: | Yonezawa Takayuki Lee Ji-Won Akazawa Hiroyuki Inagaki Masahiko Cha Byung-Yoon Nagai Kazuo Yagasaki Kazumi Akihisa Toshihiro Woo Je-Tae |
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Institution: | a Department of Nutriproteomics, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan b Research Institute for Biological Functions, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan c College of Science and Technology, Nihon University, Tokyo 101-8308, Japan d National Institute of Advanced Industrial Science and Technology (AIST), Aichi 463-8560, Japan e Division of Applied Life Science, Tokyo Noko University, Tokyo 183-8509, Japan |
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Abstract: | Osteogenic activity of six diarylheptanoids, acerogenin A (1), (R)-acerogenin B (2), aceroside I (3), aceroside B1 (4), aceroside III (5) and (−)-centrolobol (6) and two phenolic compounds; (+)-rhododendrol (7) and (+)-cathechin (8), isolated from the stem bark of Acer nikoense (Nikko maple) was evaluated using alkaline phosphatase (ALP) activity as a marker for early osteoblast differentiation. We found that the diphenyl ether-type cyclic diarylheptanoids 1-5 promoted ALP activity in mouse preosteoblastic MC3T3-E1 cells without affecting cell proliferation, but linear-type diarylheptanoid 6 and phenolic compounds 7 and 8 did not. Diphenyl ether-type cyclic diarylheptanoids 1-4 also increased protein production of osteocalcin, a late stage maker for osteoblast differentiation, and induced osteoblastic mineralization. Structure-activity relationships of these compounds demonstrated that the stimulative efficacy of aglycones was higher than that of its glycosides. Taken together, diphenyl ether-type cyclic diarylheptanoids promote early- and late-stage osteoblastogenesis, which may open the possibility for the development of novel osteogenic agents. |
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Keywords: | Acer nikoense Diarylheptanoids Osteoblast Differentiation Mineralization |
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