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Robust in-gel fluorescence detection of mucin-type O-linked glycosylation |
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| Authors: | Zaro Balyn W Bateman Leslie A Pratt Matthew R |
| Affiliation: | a Department of Chemistry, University of Southern California, Los Angeles, CA 90089, USA b Department of Molecular and Computational Biology, University of Southern California, Los Angeles, CA 90089, USA |
| Abstract: | Mucin-type O-linked glycosylation is a common post translational modification of cell-surface and secretory pathway proteins and is implicated in vital biological processes as well as human disease. We report here the use of the metabolic chemical reporter GalNAz along with Cu(I)-catalyzed [3+2] azide-alkyne cycloaddition conditions for the robust, in-gel fluorescent visualization of mucin-type O-linked glycoproteins. |
| Keywords: | Glycosylation Post-translational modification Chemical reporter Click chemistry Mucin-type O-Linked |
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