Characterization of microsatellite loci developed for Amaranthus hypochondriacus and their cross-amplifications in wild species |
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| Authors: | Jung-Ro Lee Gi-Yun Hong Anupam Dixit Jong-Wook Chung Kyung-Ho Ma Jae-Hak Lee Hee-Kyoung Kang Yang-Hee Cho Jae-Gyun Gwag Yong-Jin Park |
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| Affiliation: | (1) Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA, 225, Seodun-dong, Suwon, Kyunggi, 441-707, Republic of Korea;(2) Department of Agronomy, Graduate school, Chungbuk National University, Chengju, Chungbuk, 361-763, Republic of Korea;(3) Korea Plant Resource Institute, Paju, Kyunggi, 413-832, Republic of Korea;(4) College of Industrial Science, Kongju National University, Yesan, 340-802, Republic of Korea;(5) Bioversity International-APO Regional Office, P.O. Box 236, UPM Post Office, Serdang, Selangor, 43400, Malaysia |
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| Abstract: | The present study reports isolation and characterization of 12 polymorphic microsatellite markers for Amaranthus hypochondriacus. A total of 92 alleles were detected across the 20 accessions, with an average of 7.7 alleles per locus. The observed (H O ) and expected (H E ) heterozygosity values ranged from 0 to 0.95 and from 0.49 to 0.92, respectively. At significance threshold (P < 0.05), nine loci deviated from Hardy-Weinberg equilibrium (HWE), whereas significant linkage disequilibria (LD) were observed between five pairs of loci. The 12 loci were successfully amplified in 18 other amaranth species representing cultivated grain and vegetable species, their putative progenitors and wild species. These results demonstrate wide potential applicability of these markers for the study of intra- and inter-specific genetic diversity as well as evolutionary relationships among cultivated and wild amaranths. |
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| Keywords: | Amaranths Conservation Evolutionary relationship Genetic diversity Microsatellite markers |
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